Mass spectrometry is an analytical technique that has undergone a great technological development in recent years. This technique allows the study of compounds of diverse nature: organic, inorganic or biological and obtain qualitative or quantitative information. By mass spectrometry analysis is possible to obtain information about the molecular mass of test compound and the same structural information. This requires ionize molecules and get the ions formed in the gas phase. This process takes place in the ionization source and currently, there are different techniques to carry out like electron impact (EI), fast atom bombardment (FAB), atmospheric pressure chemical ionization (APCI), Desorption / Ionization-Assisted Laser by matrix (MALDI) or electrospray (ESI). The ions generated are accelerated to an analyzer and separated according to their mass / charge relation (m/z) by applying electric fields, magnetic fields or by determining the time of arrival at the detector. Ions reaching the detector produces an electrical signal that is processed, amplified and sent to a computer. The record obtained is called Mass spectrum and ion abundances is obtained as a function of mass / charge ratio of ions detected.
The Department of Mass Spectrometry of the Chemical Research Institute has an analyzer Bruker Esquire 6000 Ion Trap with ESI and APCI sources, able to determine relations m/z up to 6000 Dalton